Journals of Gerontology Series A: Biological Sciences and Medical Sciences Large Type Edition
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]
Author:
 		Keyword(s):
Year:  Vol:  Page: 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Menshikova, E. V.
Right arrow Articles by Goodpaster, B. H.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Menshikova, E. V.
Right arrow Articles by Goodpaster, B. H.
The Journals of Gerontology Series A: Biological Sciences and Medical Sciences 61:534-540 (2006)
© 2006 The Gerontological Society of America

Effects of Exercise on Mitochondrial Content and Function in Aging Human Skeletal Muscle

Elizabeth V. Menshikova, Vladimir B. Ritov, Liane Fairfull, Robert E. Ferrell, David E. Kelley and Bret H. Goodpaster

1 Division of Endocrinology and Metabolism, Department of Medicine, University of Pittsburgh School of Medicine, and 2 Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pennsylvania.

Address correspondence to Bret H. Goodpaster, PhD, University of Pittsburgh, 3459 Fifth Avenue, MUH N807, Pittsburgh, PA 15213. E-mail: goodpaster{at}dom.pitt.edu

Skeletal muscle mitochondria are implicated with age-related loss of function and insulin resistance. We examined the effects of exercise on skeletal muscle mitochondria in older (age = 67.3 ± 0.6 years) men (n = 5) and women (n = 3). Similar increases in (p <.01) cardiolipin (88.2 ± 9.0 to 130.6 ± 7.5 µg/mU creatine kinase activity [CK]) and the total mitochondrial DNA (1264 ± 170 to 1895 ± 273 copies per diploid of nuclear genome) reflected increased mitochondria content. Succinate oxidase activity, complexes 2–4 of the electron transport chain (ETC), increased from 0.13 ± 0.02 to 0.20 ± 0.02 U/mU CK (p <.01). This improvement was more pronounced (p <.05) in subsarcolemmal (127 ± 48%) compared to intermyofibrillar (56 ± 12%) mitochondria. NADH oxidase activity, representing total ETC activity, increased from 0.51 ± 0.09 to 1.00 ± 0.09 U/mU CK (p <.01). In conclusion, exercise enhances mitochondria ETC activity in older human skeletal muscle, particularly in subsarcolemmal mitochondria, which is likely related to the concomitant increases in mitochondrial biogenesis.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
All GSA journals The Gerontologist
Journals of Gerontology Series B: Psychological Sciences and Social Sciences
Copyright © 2006 by The Gerontological Society of America.