Journals of Gerontology Series A: Biological Sciences and Medical Sciences Large Type Edition
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The Journals of Gerontology Series A: Biological Sciences and Medical Sciences 59:B411-B415 (2004)
© 2004 The Gerontological Society of America

Aging of Human Epidermis: Reversal of Aging Changes Correlates With Reversal of Keratinocyte Fas Expression and Apoptosis

Amos Gilhar1,, Yehuda Ullmann1, Rachel Karry2, Raya Shalaginov1, Bedia Assy1, Sima Serafimovich1 and Richard S. Kalish3,

1 Skin Research Laboratory, The B. Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, and Flieman Medical Center, Haifa, Israel.
2 Laboratory of Psychology, Department of Psychiatry, Rambam Medical Center and Rappaport Faculty of Medicine, Technion, Israel.
3 Department of Dermatology, State University of NewYork at Stony Brook.

Additional correspondence to Dr. Amos Gilhar, Laboratory for Skin Research, Rappaport Building, Technion Faculty of Medicine, POB 9649, Bat-Galim, Haifa, 31096, Israel. E-mail: gilhar{at}techunix.technion.ac.il

Address correspondence to Dr. Richard S. Kalish, Department of Dermatology, Health Sciences Center T-16, 060, SUNY at Stony Brook, Stony Brook, NY 11794-8165. E-mail: richard.kalish{at}stonybrook.edu

The goal of this study was to determine the role of Fas-mediated apoptosis in human epidermal aging. Epidermal Fas expression and apoptosis are increased in aged human skin. Aging changes of human epidermis, including decreased epidermal thickness and proliferation, are reversed following grafting of human skin to SCID (severe combined immunodeficiency) mice. Skin from aged participants (n = 14; mean 70.7 years), and young participants (n = 14; mean 23.4 years) was grafted to beige SCID mice, and epidermal thickness, proliferation (Ki-67 expression), apoptosis (TUNEL [Tdt-mediated dUTP nick end labeling] reaction below granular layer), and expression of Fas and FasL were determined by histology and immunochemical staining. Aged skin was associated with thinning of the epidermis, decreased epidermal proliferation, a significant increase in apoptosis below the granular layer, and epidermal Fas expression. Engraftment significantly reversed these aging changes, including apoptosis, and Fas expression. Correlation of reversal of aging changes, with decreased epidermal Fas expression and apoptosis, supports a role for Fas-mediated apoptosis in aging of human epidermis.






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