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Glyceraldehyde-3-Phosphate Dehydrogenase Varies With Age in Glycolytic Muscles of Rats

^a Department of Anatomy, College of Medicine
^b Institute on Aging, University of South Florida, Tampa

Stephen E. Alway, Division of Exercise Physiology, Department of Human Performance and Applied Exercise Science, School of Medicine, West Virginia University, P.O. Box 9227, Morgantown, WV 26506-9227 E-mail: salway{at}wvu.edu.

Decision Editor: Jay Roberts, PhD

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA, protein, and enzyme activity levels in hindlimb muscles of adult and senescent Fischer 344 x Brown Norway rats were investigated. Soleus muscles from adult and senescent rats had similar levels of GAPDH. In contrast, muscles containing a large proportion of glycolytic fibers had lower GAPDH levels in senescent rats relative to these muscles in adult rats; this was observed at both the mRNA and protein levels. These data indicate that skeletal muscle glycolytic capacity of fast muscles is diminished with age and that it may be caused by changes at the level of transcription. Also, because GAPDH mRNA levels change with age in several rat muscles, GAPDH mRNA is not always a proper internal control for mRNA analyses of aging skeletal muscle.

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