Journals of Gerontology Series A: Biological Sciences and Medical Sciences, Vol 54, Issue 8 B318-B323, Copyright © 1999 by The Gerontological Society of America

JOURNAL ARTICLE

The age-related accumulation of protein carbonyl in rat liver correlates with the age-related decline in liver proteolytic activities

S Vittorini, C Paradiso, A Donati, G Cavallini, M Masini, Z Gori, M Pollera and E Bergamini
Istituto di Patologia Generale, University of Pisa, Italy.

Increases of protein carbonyl in animal tissues have been associated with the aging process. So far, the accumulation of oxidized proteins, highly susceptible to proteolysis, has been attributed to age-related changes in proteasomal alkaline proteases. Carbonyl in protein was monitored in six different tissues of male Sprague-Dawley rats fed ad libitum up to the age of 27 months, and of 24 and 27-month-old rats subjected to anti-aging diet restriction (every-other-day feeding ad libitum). Alkaline protease activities and liver lysosomal proteolysis were studied. The levels of protein carbonyl were significantly different in different tissues, and quite stable throughout life; accumulation was restricted to liver tissue very late in life, between ages 24 and 27 months; was fully prevented by diet restriction; was not accompanied by any diet-restriction-sensitive decline of alkaline protease activity; and was accompanied by a dramatic age-related decline in lysosomal proteolysis that was partially prevented by anti- aging diet restriction. No correlation was found between levels of alkaline protease activity and levels of protein carbonyl in the different tissues from younger animals. It is concluded that the process of autophagy, a well-known mechanism for cell maintenance, may deserve more interest in aging studies.

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