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Journals of Gerontology Series A: Biological Sciences and Medical Sciences, Vol 52, Issue 6 B318-B330, Copyright © 1997 by The Gerontological Society of America
JOURNAL ARTICLE |
SE Fleming and R Gill
Department of Nutritional Sciences, University of California, Berkeley, USA.
Metabolism was studied in colonocytes isolated from young (4 mo) and aged (24 mo) Fischer 344 rats. Animals were fed fiber-free, low-fiber (5% cellulose), or high-fiber (oat bran or NIH 31 stock) diets. Colonocytes isolated from aged animals oxidized both short- and long- chain fatty acids at significantly higher rates than did colonocytes isolated from young animals. No differences between the young and aged were noted for the oxidation to CO2 of glucose and glutamine or for flux of glucose through glycolysis. Net adenosine triphosphate (ATP) production by colonocytes was calculated to be 20% higher for the aged than for the young, although the relative contribution of substrates to net ATP production from exogenous substrates was similar for the young and aged (45-50% from butyrate, 20-25% from glucose, and 30% from other substrates including acetate, propionate, palmitate, and glutamine). Substrate oxidation was generally higher in colonocytes from the oat bran (17% total dietary fiber, highly soluble fiber) versus fiber-free diet.
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K. L. Zambell, M. D. Fitch, and S. E. Fleming Acetate and Butyrate Are the Major Substrates for De Novo Lipogenesis in Rat Colonic Epithelial Cells J. Nutr., November 1, 2003; 133(11): 3509 - 3515. [Abstract] [Full Text] [PDF] |
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J. D. Cremin Jr., M. D. Fitch, and S. E. Fleming Glucose alleviates ammonia-induced inhibition of short-chain fatty acid metabolism in rat colonic epithelial cells Am J Physiol Gastrointest Liver Physiol, June 9, 2003; 285(1): G105 - 114. [Abstract] [Full Text] [PDF] |
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J R Jorgensen, M D Fitch, P B Mortensen, and S E Fleming Absorption and metabolism of octanoate by the rat colon in vivo: concentration dependency and influence of alternative fuels Gut, July 1, 2002; 51(1): 76 - 81. [Abstract] [Full Text] [PDF] |
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J. Jorgensen and P. B. Mortensen Substrate utilization by intestinal mucosal tissue strips from patients with inflammatory bowel disease Am J Physiol Gastrointest Liver Physiol, August 1, 2001; 281(2): G405 - 411. [Abstract] [Full Text] [PDF] |
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M. D. Fitch and S. E. Fleming Metabolism of short-chain fatty acids by rat colonic mucosa in vivo Am J Physiol Gastrointest Liver Physiol, July 1, 1999; 277(1): G31 - 40. [Abstract] [Full Text] |
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