Journals of Gerontology Series A: Biological Sciences and Medical Sciences, Vol 52, Issue 6 B311-B317, Copyright © 1997 by The Gerontological Society of America

JOURNAL ARTICLE

Ethanol activates the interleukin-6 promoter in a human bone marrow stromal cell line

ET Keller, J Zhang and WB Ershler
Glennan Center for Geriatrics and Gerontology, Eastern Virginia Medical School, Norfolk, USA.

Chronic ethanol consumption is associated with the development of osteoporosis. The pro-inflammatory cytokine interleukin-6 (IL-6) plays a role in the development of osteoporosis through stimulation of osteoclastic activity. We hypothesized that ethanol promotes osteoporosis, in part, by increasing IL-6 production in the bone microenvironment. Accordingly, we evaluated ethanol's effect on IL-6 production in the Saka human bone marrow stromal cell line and in the HOBIT human osteoblast-like cell line. We found that ethanol increased IL-6 protein levels in the culture supernatants from Saka, but not HOBIT, cells. In addition, we observed that ethanol increased steady- state IL-6 mRNA levels and activated an IL-6 promoter-driven reporter vector in Saka cells. We conclude that ethanol stimulates IL-6 expression in the Saka bone marrow stromal cell line by enhancing transcriptional activity of the IL-6 gene. Our findings support the contention that ethanol may contribute to the pathogenesis of osteoporosis, in part, by increasing IL-6 expression in the bone microenvironment.

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				J. Dai, D. Lin, J. Zhang, P. Habib, P. Smith, J. Murtha, Z. Fu, Z. Yao, Y. Qi, and E. T. Keller Chronic alcohol ingestion induces osteoclastogenesis and bone loss through IL-6 in mice J. Clin. Invest., October 1, 2000; 106(7): 887 - 895. [Abstract] [Full Text]