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Journals of Gerontology Series A: Biological Sciences and Medical Sciences, Vol 51, Issue 2 B111-B116, Copyright © 1996 by The Gerontological Society of America
JOURNAL ARTICLE |
SL Mader, CL Downing, J Amos-Landgraf and P Swebjka
VA Medical Center and Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
Blood vessels from aged animals and humans have impaired relaxation to beta-adrenergic stimulation. We hypothesized that a loss of stimulatory G protein (Gs) or an increase in inhibitory G proteins (Gi) could explain this impairment. Aortic membranes from Fischer 344 rats of 4 age groups (6 week to 24 month) were studied. G-protein levels were initially assessed using cholera and pertussis toxin labeling. There was a marked decline in cholera toxin labeling (which primarily labels Gs alpha) from 6 weeks to 6 months which persisted in 12-month and 24- month animals. Pertussis toxin labeling (which primarily labels Gi alpha) showed only a slight decline with age. Western blotting was performed using specific antibodies for the alpha subunit of Gs, Gi1&2, Gi3, and G beta. There was no significant change in Gs alpha, Gi alpha, or G beta protein levels with age. We conclude there is a loss of cholera toxin-catalyzed ADP ribosylation with age, which does not represent a loss of the stimulatory alpha subunit of G protein. These data suggest that the loss of cholera toxin labeling seen with age may be a marker for loss of Gs alpha function.
This article has been cited by other articles: (Search Google Scholar for Other Citing Articles)
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  W. E. Schutzer, J. F. Reed, and S. L. Mader Decline in caveolin-1 expression and scaffolding of G protein receptor kinase-2 with age in Fischer 344 aortic vascular smooth muscle Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2457 - H2464. [Abstract] [Full Text] [PDF]
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 B. Bouscarel, Y. Matsuzaki, M. Le, T. W. Gettys, and H. Fromm Changes in G protein expression account for impaired modulation of hepatic cAMP formation after BDL Am J Physiol Gastrointest Liver Physiol, June 1, 1998; 274(6): G1151 - 1159. [Abstract] [Full Text] [PDF]
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 W. E. Schutzer, J. F. Reed, M. Bliziotes, and S. L. Mader Upregulation of G protein-linked receptor kinases with advancing age in rat aorta Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2001; 280(3): R897 - 903. [Abstract] [Full Text]
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