Journals of Gerontology Series A: Biological Sciences and Medical Sciences Large Type Edition
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The Journals of Gerontology Series A: Biological Sciences and Medical Sciences 63:21-34 (2008)
© 2008 The Gerontological Society of America

Pathways Change in Expression During Replicative Aging in Saccharomyces cerevisiae

Gloria Yiu, Alejandra McCord, Alison Wise, Rishi Jindal, Jennifer Hardee, Allen Kuo, Michelle Yuen Shimogawa, Laty Cahoon, Michelle Wu, John Kloke, Johanna Hardin and Laura L. Mays Hoopes

Departments of 1 Biology and 2 Mathematics, Pomona College, Claremont, California.

Address correspondence to Laura L. Mays Hoopes, PhD, Biology Department, Pomona College, 609 N. College Ave., Claremont, CA 91711. E-mail: lhoopes{at}pomona.edu

Yeast replicative aging is a process resembling replicative aging in mammalian cells. During aging, wild-type haploid yeast cells enlarge, become sterile, and undergo nucleolar enlargement and fragmentation; we sought gene expression changes during the time of these phenotypic changes. Gene expression studied via microarrays and quantitative real-time reverse-transcription polymerase chain reaction (qPCR) has shown reproducible, statistically significant changes in messenger RNA (mRNA) of genes at 12 and 18–20 generations. Our findings support previously described changes towards aerobic metabolism, decreased ribosome gene expression, and a partial environmental stress response. Our findings include a pseudostationary phase, downregulation of methylation-related metabolism, increased nucleotide excision repair–related mRNA, and a strong upregulation of many of the regulatory subunits of protein phosphatase I (Glc7). These findings are correlated with aging changes in higher organisms as well as with the known involvement of protein phosphorylation states during yeast aging.

Key Words: Replicative aging • Saccharomyces cerevisiae • Gene expression






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