This Article Full Text Full Text (PDF) Services Download to citation manager Citing Articles Citing Articles via HighWire PubMed PubMed Citation

Impact of Aging on Rat Bone Marrow-Derived Stem Cell Chondrogenesis

¹ University of Iowa Department of Orthopaedics and Rehabilitation, Iowa City.
² Department of Surgery, Medical University of Ohio, Toledo.
³ North Dakota College of Medicine and Health Sciences, Grand Forks.

Address correspondence to Joseph A. Buckwalter, MD, 01008 Pappajohn Pavilion, Department of Orthopaedics, University of Iowa College of Medicine, Iowa City, IA 52242. E-mail: joseph-buckwalter{at}uiowa.edu

Damaged articular cartilage rarely heals or regenerates in middle-aged and elderly adults, suggesting that the chondrogenic potential of mesenchymal stem cells declines with age. To test this hypothesis, we measured the responses of rat bone marrow-derived mesenchymal stem cells (BMSCs) to chondrogenic induction in vitro. BMSCs from immature rats (1 week old), young adult rats (12 weeks old), and old adult rats (1 year old) were analyzed for cartilage extracellular matrix (ECM) production. Histologic analysis showed strong cartilage ECM formation by BMSCs from 1-week-old rats, but not by BMSCs from 12-week-old or 1-year-old rats. Real-time polymerase chain reaction revealed age-related declines in messenger RNA encoding type II collagen, aggrecan, and link protein, three major cartilage ECM components. Microarray analysis indicated significant age-related differences in the expression of genes that influence cartilage ECM formation. These findings support the hypothesis that the chondrogenic potential of mesenchymal stem cells declines with age.

This article has been cited by other articles:

				G. M. Hoben, E. J. Koay, and K. A. Athanasiou Fibrochondrogenesis in Two Embryonic Stem Cell Lines: Effects of Differentiation Timelines Stem Cells, February 1, 2008; 26(2): 422 - 430. [Abstract] [Full Text] [PDF]

				H. R. Warner 2006 Kent Award Lecture: Is Cell Death and Replacement a Factor in Aging? J. Gerontol. A Biol. Sci. Med. Sci., November 1, 2007; 62(11): 1228 - 1232. [Abstract] [Full Text] [PDF]