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Physicochemical Composition of Osteoporotic Bone in the Trichothiodystrophy Premature Aging Mouse Determined by Confocal Raman Microscopy

Departments of ¹ Polymer Chemistry and Biomaterials and ⁴ Biophysical Techniques, Faculty of Technology and Sciences, University of Twente, Bilthoven, The Netherlands.
² Department of Carcinogenesis, Mutagenesis and Aging, The National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands.
³ University Medical Center Rotterdam, Department of Genetics, The Netherlands.

Address correspondence to A. A. van Apeldoorn, PhD BioSc, Department of Polymer Chemistry and Biomaterials, Faculty of Technology and Sciences, University of Twente, P.O. Box 98, 3720 AB Bilthoven, The Netherlands. E-mail: a.a.vanapeldoorn{at}tnw.utwente.nl

Although it has been established that premature aging trichothiodystrophy (TTD) mice display typical signs of osteoporosis, exact changes in physicochemical properties of these mice have not been elucidated. We used confocal Raman microscopy and histology to study femora of TTD mice. We measured femora isolated from xeroderma pigmentosum group A (XPA)/TTD double mutant mice to establish that Raman microscopy can be applied to measure differences in bone composition. Raman data from XPA/TTD mice showed remarkable changes in bone mineral composition. Moreover, we observed a severe form of osteoporosis, with strongly reduced cortical bone thickness. We used Raman microscopy to analyze bone composition in eight wild-type and eight TTD animals, and observed decreased levels of phosphate and carbonate in the cortex of femora isolated from TTD mice. In contrast, the bands representing the bone protein matrix were not affected in these mice.

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