HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Argentino, D. P. Articles by Turyn, D. Search for Related Content PubMed PubMed Citation Articles by Argentino, D. P. Articles by Turyn, D.

Effects of Long-Term Caloric Restriction on Early Steps of the Insulin-Signaling System in Mouse Skeletal Muscle

¹ Instituto de Química y Fisicoquímica Biológicas (UBA-CONICET), Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
² Departments of Physiology, Medicine, and Pharmacology, School of Medicine, Southern Illinois University, Springfield.

Address correspondence to Daniel Turyn, Instituto de Química y Fisicoquímica Biológicas (IQUIFIB), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junin 956 (1113), Buenos Aires, Argentina. E-mail: dturyn{at}qb.ffyb.uba.ar

In this study, we analyzed the effects of long-term (14 months) caloric restriction (CR) on the first steps of the insulin signaling system in skeletal muscle of normal mice. CR induced a significant decrease in serum insulin and glucose levels, indicating an enhancement of insulin sensitivity. CR reduced the in vivo insulin-induced phosphorylation of the insulin receptor substrate (IRS)-1 by 27%, but this difference was not significant (p =.298). CR reduced insulin receptor (IR) abundance by 34% from the ad libitum values, but this difference did not reach significance (p =.246). The abundance of the p85 regulatory subunit of PI3K and glucose transporter 4 was unaltered after CR. However, IRS-1 abundance was significantly increased by 42% in muscle of mice exposed to CR. These findings indicate that the CR-induced improvement of insulin action in mice is not related to changes in glucose transporter 4, the p85 regulatory subunit of PI3K, or IR abundance in skeletal muscle but might be related to an increase in IRS-1 abundance in this tissue.

This article has been cited by other articles:

				J. F. Giani, M. S. Bonkowski, M. C. Munoz, M. M. Masternak, D. Turyn, A. Bartke, and F. P. Dominici Insulin Signaling Cascade in the Hearts of Long-Lived Growth Hormone Receptor Knockout Mice: Effects of Calorie Restriction J. Gerontol. A Biol. Sci. Med. Sci., August 1, 2008; 63(8): 788 - 797. [Abstract] [Full Text] [PDF]

				E. B. Arias and G. D. Cartee In vitro simulation of calorie restriction-induced decline in glucose and insulin leads to increased insulin-stimulated glucose transport in rat skeletal muscle Am J Physiol Endocrinol Metab, December 1, 2007; 293(6): E1782 - E1788. [Abstract] [Full Text] [PDF]

				J. F. Giani, M. M. Gironacci, M. C. Munoz, C. Pena, D. Turyn, and F. P. Dominici Angiotensin-(1 7) stimulates the phosphorylation of JAK2, IRS-1 and Akt in rat heart in vivo: role of the AT1 and Mas receptors Am J Physiol Heart Circ Physiol, August 1, 2007; 293(2): H1154 - H1163. [Abstract] [Full Text] [PDF]

				C. Selman, N. D. Kerrison, A. Cooray, M. D. W. Piper, S. J. Lingard, R. H. Barton, E. F. Schuster, E. Blanc, D. Gems, J. K. Nicholson, et al. Coordinated multitissue transcriptional and plasma metabonomic profiles following acute caloric restriction in mice Physiol Genomics, November 21, 2006; 27(3): 187 - 200. [Abstract] [Full Text] [PDF]

				Z. Wang, K. A. Al-Regaiey, M. M. Masternak, and A. Bartke Adipocytokines and lipid levels in ames dwarf and calorie-restricted mice. J. Gerontol. A Biol. Sci. Med. Sci., April 1, 2006; 61(4): 323 - 331. [Abstract] [Full Text] [PDF]