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The Journals of Gerontology Series A: Biological Sciences and Medical Sciences 58:B205-B211 (2003)
© 2003 The Gerontological Society of America

Age-related Base Excision Repair Activity in Mouse Brain and Liver Nuclear Extracts

Gabriel W. Intano1, Eun Ju Cho1, C. Alex McMahan2 and Christi A. Walter1,3

1 Department of Cellular & Structural Biology
2 Department of Pathology, The University of Texas Health Science Center at San Antonio.
3 South Texas Veterans Health Care System, Audie L. Murphy Hospital, San Antonio.

To assess DNA repair activity relative to age, in vitro base excision repair assays were performed using brain and liver nuclear extracts prepared from mice of various ages. An 85% decline in repair activity was observed in brain nuclear extracts and a 50% decrease in liver nuclear extracts prepared from old mice compared with 6-day-old mice. Brain nuclear extracts prepared from old mice showed a decreased abundance of DNA polymerase-ß, but the addition of purified protein did not restore base excision repair activity. Abundances of other tested base excision repair proteins did not change relative to age. The conclusion is that, during aging, a decline in DNA repair could contribute to increased levels of DNA damage and mutagenesis.






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Copyright © 2003 by The Gerontological Society of America.